Whole-Brain Connectome of a Drosophila female brain coming soon (early 2023). AI-segmented, expert-proofread neurons with over 76 million connections, crowdsourced labels, and neurotransmitters.

State of the Connectome: Upcoming FlyWire Town Hall on March 30th at 11:00 am US ET.

Mar. 30 - Town Hall RSVP Explore The Connectome


Looking for FlyWire Proofreading?

Proofreading app is here: edit.flywire.ai


103K+ Neurons
Central brain and optic lobes proofread by experts
76M+ Connections
Including Neurotransmitter Information
144K+ Annotations
Cell labels from the FlyWire community


Since 2019, scientists and experienced proofreaders have utilized FlyWire to proofread AI segmentation of a full fly brain (Dorkenwald et al., Zheng et al.). As of October, 2022, over 80,000 neurons have been proofread in FlyWire, including the entire central brain.

Automatically extracted presynaptic and postsynaptic tags have been applied to all putative connections in the brain (Buhmann et al.), and the dominant neurotransmitter assigned for most neurons (Eckstein et al.).

Explore networks using neuron IDs in the FlyWire Codex (FlyWire signup required). Further analysis tools are forthcoming. For programmatic analysis, see natverse (R) and CAVE (Python).

Drosophila Melanogaster, connectome
Brain Initiative

FlyWire is created by the labs of Mala Murthy and Sebastian Seung at Princeton University. It is funded by the US Brain Initiative. Proofreading and annotation has been carried out in collaboration with the Cambridge Drosophila Connectomics Group (funded by the Wellcome trust) and many other labs around the world.

Proofreading Completion Status

Central Brain Proofreading Completion


Optic Lobes Proofreading Completion


Overall Proofreading Completion


Citing guidelines for publishing with FlyWire

Scientific publications derived in whole or in part from use of FlyWire should cite FlyWire: online community for whole brain connectomics. Dorkenwald et al. Nature Methods 2022, and should acknowledge the Princeton FlyWire team and members of the Murthy and Seung labs for development and maintenance of FlyWire (supported by BRAIN Initiative grant MH117815 to Murthy and Seung).

To use neurons proofread of labeled by the community, see this document.

Publications Utilizing FlyWire

  • Structured sampling of olfactory input by the fly mushroom body. Zheng et al. Current Biology 2022
  • Neural network organization for courtship-song feature detection in Drosophila. Baker et al. Current Biology 2022
  • Taste quality and hunger interactions in a feeding sensorimotor circuit. Shiu et al. eLife 2022
  • The neural basis for a persistent internal state in Drosophila females. Deutsch et al. eLife 2022
  • Chemoreceptor co-expression in Drosophila melanogaster olfactory neurons. Task et al. eLife 2022
  • Mating-driven variability in olfactory local interneuron wiring. Chou et al. Science Advances 2022
  • Olfactory stimuli and moonwalker SEZ neurons can drive backward locomotion in Drosophila. Israel et al. Current Biology 2022
  • Synaptic targets of photoreceptors specialized to detect color and skylight polarization in Drosophila. Kind et al. eLife 2021
  • Classification and genetic targeting of cell types in the primary taste and premotor center of the adult Drosophila brain. Stern et al. eLife 2021
  • Information flow, cell types and stereotypy in a full olfactory connectome. Schlegel et al. eLife 2021