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FlyWire
FlyWire
Whole-Brain Connectome of a Drosophila female brain. AI-segmented, expert-proofread neurons with millions of connections, crowdsourced labels, and neurotransmitters.

Explore the Connectome in Codex

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AT A GLANCE

120K+ Neurons
Central brain and optic lobes proofread by experts
30M+ Synapses
Including Neurotransmitter Information
100K+ Annotations
Cell labels from the FlyWire community
Buildings

Overview

Since 2019, scientists and experienced proofreaders have utilized FlyWire to proofread AI segmentation of a full fly brain (Dorkenwald et al., Zheng et al.). As of July 2023, the FlyWire flagship preprint Neuronal wiring diagram of an adult brain is available on bioRxiv, which includes 127,978 proofread neurons (Dorkenwald et al.). The companion annotation paper includes over 700,000 labels (Schlegel et al.).

Automatically extracted presynaptic and postsynaptic tags have been applied to all putative connections in the brain (Buhmann et al.), and the dominant neurotransmitter assigned for most neurons (Eckstein et al.).

Explore the connectome and its annotations in Codex.

Drosophila Melanogaster, connectome

FlyWire Consortium

Community of neurobiologists, computer scientists, and proofreaders who build and curate the first whole brain connectome for Drosophila in FlyWire. Join FlyWire and contribute community data for your lab to appear.

Meet the Consortium

Brain Initiative

FlyWire is created by the labs of Mala Murthy and Sebastian Seung at Princeton University. It is funded by the US Brain Initiative. Proofreading and annotation has been carried out in collaboration with the Cambridge Drosophila Connectomics Group (funded by the Wellcome trust) and many other labs around the world.




Publications Utilizing FlyWire (see citation guidelines)

  • Neuronal wiring diagram of an adult brain. Dorkenwald et al. bioRxiv 2023
  • Whole-brain annotation and multi-connectome cell typing quantifies circuit stereotypy in Drosophila. Schlegel et al. bioRxiv 2023
  • Neuronal "parts list" and wiring diagram for a visual system. Matsliah et al. bioRxiv 2023
  • Overlap and divergence of neural circuits mediating distinct behavioral responses to sugar. Jacobs et al. bioRxiv 2023
  • Synaptic and peptidergic connectomes of the Drosophila circadian clock. Reinhard et al. bioRxiv 2023
  • Neuronal correlates of time integration into memories. Frantzmann et al. bioRxiv 2023
  • Networks of descending neurons transform command-like signals into population-based behavioral control. Braun et al. bioRxiv 2023
  • Descending control and regulation of spontaneous flight turns in Drosophila. Ros et al. bioRxiv 2023
  • A leaky integrate-and-fire computational model based on the connectome of the entire adult Drosophila brain reveals insights into sensorimotor processing. Shiu et al. bioRxiv 2023
  • Hunger- and thirst-sensing neurons modulate a neuroendocrine network to coordinate sugar and water ingestion. González-Segarra et al. bioRxiv 2023
  • Disynaptic inhibition shapes tuning of OFF-motion detectors in Drosophila. Braun et al. Current Biology 2023
  • Hue selectivity from recurrent circuitry in Drosophila. Christenson et al. bioRxiv 2023
  • Neurotransmitter Classification from Electron Microscopy Images at Synaptic Sites in Drosophila Melanogaster. Eckstein et al. bioRxiv 2023
  • Visual Feedback Neurons Fine-Tune Drosophila Male Courtship via GABA-Mediated Inhibition. Mabuchi et al. bioRxiv 2023
  • Somatotopic organization among parallel sensory pathways that promote a grooming sequence in Drosophila. Eichler et al. eLife 2023
  • Neural network organization for courtship-song feature detection in Drosophila. Baker et al. Current Biology 2023
  • Eye structure shapes neuron function in Drosophila motion vision. Zhao et al. bioRxiv 2022
  • Structured sampling of olfactory input by the fly mushroom body. Zheng et al. Current Biology 2022
  • Taste quality and hunger interactions in a feeding sensorimotor circuit. Shiu et al. eLife 2022
  • Mating-driven variability in olfactory local interneuron wiring. Chou et al. Science Advances 2022
  • Olfactory stimuli and moonwalker SEZ neurons can drive backward locomotion in Drosophila. Israel et al. Current Biology 2022
  • Chemoreceptor co-expression in Drosophila melanogaster olfactory neurons. Task et al. eLife 2022
  • Synaptic targets of photoreceptors specialized to detect color and skylight polarization in Drosophila. Kind et al. eLife 2021
  • Classification and genetic targeting of cell types in the primary taste and premotor center of the adult Drosophila brain. Sterne et al. eLife 2021
  • Information flow, cell types and stereotypy in a full olfactory connectome. Schlegel et al. eLife 2021
  • The neural basis for a persistent internal state in Drosophila females. Deutsch et al. eLife 2020 (2022)


Contacts

  • Sven M. Dorkenwald (svenmd@princeton.edu), FlyWire Proofreading Platform Lead
  • Arie Matsliah (arie@princeton.edu), Research Scientist and FlyWire Data Evangelist
  • Amy Sterling (amysterling@princeton.edu), Crowdsourcing and Outreach Manager
  • Mala Murthy (mmurthy@princeton.edu), Principal Investigator
  • Sebastian Seung (sseung@princeton.edu), Principal Investigator