FlyWire

AT A GLANCE

120K+ Neurons
Central brain and optic lobes proofread by experts

75M+ Connections
Including Neurotransmitter Information

50K+ Annotations
Cell labels from the FlyWire community

Overview

Since 2019, scientists and experienced proofreaders have utilized FlyWire to proofread AI segmentation of a full fly brain (Dorkenwald et al., Zheng et al.). As of April 2023, over 120,000 neurons have been proofread in FlyWire, including the entire central brain.

Automatically extracted presynaptic and postsynaptic tags have been applied to all putative connections in the brain (Buhmann et al.), and the dominant neurotransmitter assigned for most neurons (Eckstein et al.).

Explore the connectome and its annotations in Codex (FlyWire signup required). For programmatic analysis, see natverse (R) and CAVE (Python).

FlyWire Consortium

Community of neurobiologists, computer scientists, and proofreaders who build and curate the first whole brain connectome for Drosophila in FlyWire. Join FlyWire and contribute community data for your lab to appear.

Members adhere to a set of shared principles to join the community: FlyWire Data and Principles.

Meet the Consortium

FlyWire is created by the labs of Mala Murthy and Sebastian Seung at Princeton University. It is funded by the US Brain Initiative. Proofreading and annotation has been carried out in collaboration with the Cambridge Drosophila Connectomics Group (funded by the Wellcome trust) and many other labs around the world.

Proofreading Completion Status

Central Brain Proofreading Completion

95%

Optic Lobes Proofreading Completion

87%

Overall Proofreading Completion

95%

GALLERY

Some cool 3D renderings

CT1 neurons identified by Zhihao Zheng and proofread by Seung Lab. Rendered by Amy Sterling for FlyWire

APL Neurons of the mushroon body. With a combined length of 13 cm, this spectacular pair of cells is the longest in the fly brain and if laid end to end would be 43 times longer than the entire fly. Identified by Zhihao Zheng and proofread through over 3,000 edits by Jefferis, Murthy, Seung Labs. Rendered by Amy Sterling for FlyWire.

Bolt Neurons identified and proofread by Salil Bidaye and rendered by Amy Sterling for FlyWire.

Moonwalker neurons identified and proofread by Salil Bidaye and Marieke Selcho. Rendered by Amy Sterling for FlyWire.

Keystone neurons (il3LN6) identified by Philipp Schlegel of Jefferis Lab and proofread in FlyWire by labs of Jefferis, Lee, Murthy, and Seung. Rendered by Amy Sterling for FlyWire.

LPSP neurons identified by Claire McKellar of Seung Lab and Dustin Garner of Sung Soo Kim Lab. Proofread by Murthy Lab. Rendered by Amy Sterling for FlyWire.

DM4 neurons identified by Emil Kind and rendered by Amy Sterling for FlyWire

AVLP538 neurons identified by Philipp Schlegel of Greg Jefferis Lab and proofread by members from Jefferis, Murthy, Seung Labs + citizen scientists AzureJay and KrzystofKruk. Rendered by Amy Sterling for FlyWire

DPM neurons identified by Markus Pleijzier and proofread through 1,554 edits from members of Jefferis, Murthy, Seung, and Wilson Labs. Rendered by Amy Sterling for FlyWire

OA-AL2i1 neurons identified by Greg Jefferis and proofread through a combined 746 edits from members of Jefferis, Murthy, Seung Labs + citizen scientists Krzystof Kruk and Nseraf. Rendered by Amy Sterling for FlyWire

Drosophila brain by Greg Jefferis. Rendered by Amy Sterling for FlyWire

Citing guidelines for publishing with FlyWire

Scientific publications derived in whole or in part from use of FlyWire should cite FlyWire: online community for whole brain connectomics. Dorkenwald et al. Nature Methods 2022, and should acknowledge the Princeton FlyWire team and members of the Murthy and Seung labs for development and maintenance of FlyWire (supported by BRAIN Initiative grant MH117815 to Murthy and Seung).
To use neurons proofread of labeled by the community, see this document.

Publications Utilizing FlyWire

Structured sampling of olfactory input by the fly mushroom body. Zheng et al. Current Biology 2022
Neural network organization for courtship-song feature detection in Drosophila. Baker et al. Current Biology 2022
Taste quality and hunger interactions in a feeding sensorimotor circuit. Shiu et al. eLife 2022
The neural basis for a persistent internal state in Drosophila females. Deutsch et al. eLife 2022
Chemoreceptor co-expression in Drosophila melanogaster olfactory neurons. Task et al. eLife 2022
Mating-driven variability in olfactory local interneuron wiring. Chou et al. Science Advances 2022
Olfactory stimuli and moonwalker SEZ neurons can drive backward locomotion in Drosophila. Israel et al. Current Biology 2022
Synaptic targets of photoreceptors specialized to detect color and skylight polarization in Drosophila. Kind et al. eLife 2021
Classification and genetic targeting of cell types in the primary taste and premotor center of the adult Drosophila brain. Stern et al. eLife 2021
Information flow, cell types and stereotypy in a full olfactory connectome. Schlegel et al. eLife 2021

Contacts

Sven M. Dorkenwald (svenmd@princeton.edu), FlyWire Proofreading Platform Lead
Arie Matsliah (arie@princeton.edu), Research Scientist and FlyWire Data Evangelist
Amy Sterling (amysterling@princeton.edu), Crowdsourcing and Outreach Manager
Mala Murthy (mmurthy@princeton.edu), Primary Investigator
Sebastian Seung (sseung@princeton.edu), Primary Investigator