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FlyWire Brain
FlyWire Brain
Whole-Brain Connectome of an adult female Drosophila. AI-segmented, expert-proofread neurons with millions of connections, crowdsourced labels, and neurotransmitters.

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AT A GLANCE

120K+ Neurons
Central brain and optic lobes proofread by experts
30M+ Synapses
Including Neurotransmitter Information
100K+ Annotations
Cell labels from the FlyWire community
Buildings

Overview

Since 2019, scientists and experienced proofreaders have utilized FlyWire to proofread AI segmentation of a full fly brain (Dorkenwald et al., Zheng et al.). As of July 2023, the FlyWire flagship preprint Neuronal wiring diagram of an adult brain is available on bioRxiv, which includes 127,978 proofread neurons (Dorkenwald et al.). The companion annotation paper includes over 700,000 labels (Schlegel et al.).

Automatically extracted presynaptic and postsynaptic tags have been applied to all putative connections in the brain (Buhmann et al.), and the dominant neurotransmitter assigned for most neurons (Eckstein et al.).

Explore the connectome and its annotations in Codex.

Drosophila Melanogaster, connectome

FlyWire Consortium

Community of neurobiologists, computer scientists, and proofreaders who build and curate the first whole brain connectome for Drosophila in FlyWire. Join FlyWire and contribute community data for your lab to appear.

Meet the Consortium

Brain Initiative

FlyWire is created by the labs of Mala Murthy and Sebastian Seung at Princeton University. It is funded by the US Brain Initiative. Proofreading and annotation has been carried out in collaboration with the Cambridge Drosophila Connectomics Group (funded by the Wellcome trust) and many other labs around the world.




Publications Utilizing FlyWire (see citation guidelines)

  • Neuronal wiring diagram of an adult brain. Dorkenwald et. al. bioRxiv 2023
  • Whole-brain annotation and multi-connectome cell typing quantifies circuit stereotypy in Drosophila. Schlegel et. al. bioRxiv 2023
  • Neuronal "parts list" and wiring diagram for a visual system. Matsliah et. al bioRxiv 2023
  • Brain rewiring during developmental transitions: A Comparative Analysis of Larva and Adult Drosophila melanogaster. Yadav et. al. bioRxiv 2024
  • Divergent neural circuits for proprioceptive and exteroceptive sensing of the Drosophila leg. Lee et. al. bioRxiv 2024
  • Morphology and synapse topography optimize linear encoding of synapse numbers in Drosophila looming responsive descending neurons.. Moreno-Sanchez et. al. bioRxiv 2024
  • Ectopic Reconstitution of a Spine-Apparatus Like Structure Provides Insight into Mechanisms Underlying Its Formation. Falahati et. al. bioRxiv 2024
  • Interneuron diversity and normalization specificity in a visual system. Seung bioRxiv 2024
  • Social state gates vision using three circuit mechanisms in Drosophila. Schretter et. al. bioRxiv 2024
  • Light and dopamine impact two circadian neurons to promote morning wakefulness. Le et. al. bioRxiv 2024
  • Neural pathways and computations that achieve stable contrast processing tuned to natural scenes. Gür et. al. bioRxiv 2024
  • Neurons underlying aggressive actions that are shared by both males and females in Drosophila. Tao et. al. bioRxiv 2024
  • Molecular and Cellular Mechanisms of Teneurin Signaling in Synaptic Partner Matching. Xu et. al. bioRxiv 2024
  • Organization of an ascending circuit that conveys flight motor state in Drosophila. Cheong et. akl. Current Biology 2024
  • Taste cells expressing Ionotropic Receptor 94e reciprocally impact feeding and egg laying in Drosophila. Guillemin et. al. bioRxiv 2024
  • Anti-diuretic hormone ITP signals via a guanylate cyclase receptor to modulate systemic homeostasis in Drosophila. Gera et. al. bioRxiv 2024
  • Network Statistics of the Whole-Brain Connectome of Drosophila. Lin et. al. National Library of Medicine 2023
  • Synaptic connectome of the Drosophila circadian clock. Reinhard et. al. bioRxiv 2023
  • Presynaptic inhibition selectively suppresses leg proprioception in behaving Drosophila. Dallmann bioRxiv 2023
  • Aminergic and peptidergic modulation of Insulin-Producing Cells in Drosophila. Held bioRxiv 2023
  • Descending control and regulation of spontaneous flight turns in Drosophila. Ros et. al. Current Biology 2023
  • Neural circuit mechanisms underlying context-specific halting in Drosophila. Sapkal et. al. bioRxiv 2023
  • Heterogeneity of synaptic connectivity in the fly visual system. Cornean et. al. Nature Communications 2023
  • Overlap and divergence of neural circuits mediating distinct behavioral responses to sugar. Jacobs et. al. bioRxiv 2023
  • Diversity of visual inputs to Kenyon cells of the Drosophila mushroom body. Ganguly et. al. bioRxiv 2023
  • A comprehensive neuroanatomical survey of the Drosophila Lobula Plate Tangential Neurons with predictions for their optic flow sensitivity. Zhao et. al. bioRxiv 2023
  • Fine-grained descending control of steering in walking Drosophila. Yang et. al. bioRxiv 2023
  • From connectome to effectome: learning the causal interaction map of the fly brain. Pospisil et. al. bioRxiv 2023
  • Insights into vision from interpretation of a neuronal wiring diagram. Seung bioRxiv 2023
  • Connectomic reconstruction predicts the functional organization of visual inputs to the navigation center of the Drosophila brain. Garner et. al. bioRxiv 2023
  • Synaptic and peptidergic connectomes of the Drosophila circadian clock. Reinhard et. al. bioRxiv 2023
  • Neuronal correlates of time integration into memories. Frantzmann et. al. bioRxiv 2023
  • Networks of descending neurons transform command-like signals into population-based behavioral control. Braun et. al. bioRxiv 2023
  • A leaky integrate-and-fire computational model based on the connectome of the entire adult Drosophila brain reveals insights into sensorimotor processing. Shiu et. al. bioRxiv 2023
  • Hunger- and thirst-sensing neurons modulate a neuroendocrine network to coordinate sugar and water ingestion. González-Segarra et. al. eLife 2023
  • Disynaptic inhibition shapes tuning of OFF-motion detectors in Drosophila. Braun et. al. Current Biology 2023
  • Hue selectivity from recurrent circuitry in Drosophila. Christenson et. al. bioRxiv 2023
  • Neurotransmitter Classification from Electron Microscopy Images at Synaptic Sites in Drosophila Melanogaster. Eckstein et. al. Cell 2023 (2024)
  • Visual Feedback Neurons Fine-Tune Drosophila Male Courtship via GABA-Mediated Inhibition. Mabuchi et. al. Current Biology 2023
  • Somatotopic organization among parallel sensory pathways that promote a grooming sequence in Drosophila. Eichler et. al. eLife 2023
  • Neural network organization for courtship-song feature detection in Drosophila. Baker et. al. Current Biology 2023
  • Eye structure shapes neuron function in Drosophila motion vision. Zhao et. al. bioRxiv 2022
  • Structured sampling of olfactory input by the fly mushroom body. Zheng et. al. Current Biology 2022
  • Taste quality and hunger interactions in a feeding sensorimotor circuit. Shiu et. al. eLife 2022
  • Mating-driven variability in olfactory local interneuron wiring. Chou et. al. Science Advances 2022
  • Olfactory stimuli and moonwalker SEZ neurons can drive backward locomotion in Drosophila. Israel Current Biology 2022
  • Chemoreceptor co-expression in Drosophila melanogaster olfactory neurons. Task et. al. eLife 2022
  • Synaptic targets of photoreceptors specialized to detect color and skylight polarization in Drosophila. Kind et. al. eLife 2021
  • Classification and genetic targeting of cell types in the primary taste and premotor center of the adult Drosophila brain. Sterne et. al. eLife 2021
  • Information flow, cell types and stereotypy in a full olfactory connectome. Schlegel et. al. eLife 2021
  • The neural basis for a persistent internal state in Drosophila females. Deutsch et. al. eLife 2020 (2022)


Contacts

  • Sven M. Dorkenwald (svenmd@princeton.edu), FlyWire Proofreading Platform Lead
  • Arie Matsliah (arie@princeton.edu), Research Scientist and FlyWire Data Evangelist
  • Amy Sterling (amysterling@princeton.edu), Crowdsourcing and Outreach Manager
  • Mala Murthy (mmurthy@princeton.edu), Principal Investigator
  • Sebastian Seung (sseung@princeton.edu), Principal Investigator