Whole-Brain Connectome of a Drosophila female brain coming soon (Summer 2023). AI-segmented, expert-proofread neurons with over 75 million connections, crowdsourced labels, and neurotransmitters.

Explore in Codex Proofread / Edit


120K+ Neurons
Central brain and optic lobes proofread by experts
75M+ Connections
Including Neurotransmitter Information
50K+ Annotations
Cell labels from the FlyWire community


Since 2019, scientists and experienced proofreaders have utilized FlyWire to proofread AI segmentation of a full fly brain (Dorkenwald et al., Zheng et al.). As of April 2023, over 120,000 neurons have been proofread in FlyWire, including the entire central brain.

Automatically extracted presynaptic and postsynaptic tags have been applied to all putative connections in the brain (Buhmann et al.), and the dominant neurotransmitter assigned for most neurons (Eckstein et al.).

Explore the connectome and its annotations in Codex (FlyWire signup required). For programmatic analysis, see natverse (R) and CAVE (Python).

Drosophila Melanogaster, connectome
Brain Initiative

FlyWire is created by the labs of Mala Murthy and Sebastian Seung at Princeton University. It is funded by the US Brain Initiative. Proofreading and annotation has been carried out in collaboration with the Cambridge Drosophila Connectomics Group (funded by the Wellcome trust) and many other labs around the world.

Proofreading Completion Status

Central Brain Proofreading Completion


Optic Lobes Proofreading Completion


Overall Proofreading Completion


Citing guidelines for publishing with FlyWire

Publications Utilizing FlyWire

  • Structured sampling of olfactory input by the fly mushroom body. Zheng et al. Current Biology 2022
  • Neural network organization for courtship-song feature detection in Drosophila. Baker et al. Current Biology 2022
  • Taste quality and hunger interactions in a feeding sensorimotor circuit. Shiu et al. eLife 2022
  • The neural basis for a persistent internal state in Drosophila females. Deutsch et al. eLife 2022
  • Chemoreceptor co-expression in Drosophila melanogaster olfactory neurons. Task et al. eLife 2022
  • Mating-driven variability in olfactory local interneuron wiring. Chou et al. Science Advances 2022
  • Olfactory stimuli and moonwalker SEZ neurons can drive backward locomotion in Drosophila. Israel et al. Current Biology 2022
  • Synaptic targets of photoreceptors specialized to detect color and skylight polarization in Drosophila. Kind et al. eLife 2021
  • Classification and genetic targeting of cell types in the primary taste and premotor center of the adult Drosophila brain. Stern et al. eLife 2021
  • Information flow, cell types and stereotypy in a full olfactory connectome. Schlegel et al. eLife 2021


  • Sven M. Dorkenwald (svenmd@princeton.edu), FlyWire Proofreading Platform Lead
  • Arie Matsliah (arie@princeton.edu), Research Scientist and FlyWire Data Evangelist
  • Amy Sterling (amysterling@princeton.edu), Crowdsourcing and Outreach Manager
  • Mala Murthy (mmurthy@princeton.edu), Primary Investigator
  • Sebastian Seung (sseung@princeton.edu), Primary Investigator